Abstract & Bio
Abstract:
Electroporation uses electric pulses to create temporary pores in cell membranes. It's like using a can opener on the cell. This physical method of gene transfer can insert nucleic acids into the cell with ease. Some engineers have created a DIY electroporation device called the Electropen, but we created the BioVolt. This device uses the same principle of electroporation but in a compact, accessible form. We have developed additional hacks to this device and We will demonstrate the utility of the BioVolt-poration device by preparing E. coli (BL21 strain) electrocompetent cells and transforming them with a recombinant plasmid expressing green fluorescent protein (GFP). And then also showcase outcomes for what is needed for use on Human Mesenchymal Stem Cells (autologous). This proves that even a homemade device can achieve significant scientific results.
Bio:
Medical device cybersecurity champion, "Big A" Airman, USN Senior Chief, soon-to-be Mustang Officer, and federal government programs expert. Husband, father of three, casual gamer, jiu-jitsu enthusiast, and devoted walker of one dachshund.